Modifying Enzymes
New England Biolabs, Inc. PreCR Repair Mix - 30 reactions
The PreCR Repair Mix is an enzyme cocktail formulated to repair damaged template DNA prior to its use in the polymerase chain reaction (PCR), microarrays or other DNA technologies. PreCR is active on a broad range of DNA damages, including those that block PCR (e.g. apurinic/apyrimidinic sites, thymine dimers, nicks and gaps) and those that are mutagenic (e.g. deaminated cytosine and 8-oxo-guanine). In addition, it will remove a variety of moieties from the 3end of DNA leaving a hydroxyl group. The PreCR Repair Mix will not repair all damages that inhibit/interfere with PCR. For example, it will not repair 8-oxo-7,8-dihydro-2'deoxyadenosines or fragmented DNA. In fact, the ligase present in the mix is very active at sealing nicks in DNA but does not ligate blunt ends or nicks near a mismatch effectively. This limits the possibility of chimeric gene formation as a product of ligation. The PreCR Repair Mix can be used in conjunction with any thermophilic polymerase.
New England Biolabs, Inc. Terminal Transferase – 2500 units
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
Worthington Biochemical Corporation CATALASE 10X10
Supplied as an aqueous solution of 2X crystallized catalase (Code: CTR without thymol) filtered through a 0.22 micron pore size membrane. Minimum of 30,000 units/ml; 10 ml/vial.
Enzyme Research Laboratories Inc Human a-Thrombin (Factor IIa),1000 U
Please use this part # if ordering a quantity of 1-9. If ordering qty 10 or more please use Fisher part # NC0704726.
Human a-Thrombin (Factor IIa),1000 U,Buffer Composition = 50 mM Sodium Citrate,0.2 M NaCl,0.1% PEG-8000,pH 6.5,Extinction Coefficient (1%) = 18.3,Molecular Weight = 37,000 daltons,Prepared from human Prothrombin by activation with Factor Xa
Discovery Life Sciences MAO B SUPERSOME
Human Monoamine Oxidase B (MAO-B) 0.5mL This activity is catalyzed by MAO-B which is expressed from human MAO-B cDNA using a baculovirus expression system
New England Biolabs, Inc. T4 Polynucleotide Kinase (3' phosphatase minus) – 1000 units
T4 Polynucleotide Kinase catalyzes the transfer and exchange of P from the gamma position of ATP to the 5 -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3'-monophosphates. This modified version exhibits full kinase activity with no 3' phosphatase activity.
Haematologic Technologies Inc H GAMMA THROMBIN 100UG
Coagulation protein, Human Thrombin Beta form, 100 micrograms, Research use only, 100 mM Sodium Phosphate, pH 6.5, store at -80 Celsius.
Diagenode Inc Hyperactive Tn5 transposase, Unloaded, 20 μl, Mg++ dependent, pH 7.5-8.0, 37-55°C
Hyperactive Tn5 transposase, Unloaded, 20 μl, Mg++ dependent, pH 7.5-8.0, 37-55°C
Athens Research And Technology Inc CATALASE HU ERYTHROCYTE 1MG
Catalase, Human Erythrocyte: 1 MG; Frozen in 50 mM Tris-HCl, pH 8.0; MW 244,000; Extinction Coefficient=1.39; Purity: > =95% by SDS-PAGE; Activity: > 50,000 u/mg protein. pH 7.0. Prepared from whole blood in the Athens Research & Tech lab.
Athens Research And Technology Inc CATHEPSIN G HU NEUTROPHL 100UG
Cathepsin G, Human Neutrophil: 100 UG; Salt-free lyophilized solid; MW 23,500; Extinction Coefficient=0.664; Activity: 2-4 units per mg protein. Purity: > =95% by SDS-PAGE.
Athens Research And Technology Inc PROTEINASE 3 NEUTROPHIL 100UG
Proteinase 3, Human Neutrophil: 100 UG; Frozen from 50 mM MES, pH 4.5 with 700 mM NaCl; MW 29,000; EC=1.0; Purity: > =95% by SDS-PAGE. Native; Purified from whole blood in the Athens Research & Technology laboratory. Serine Proteinase, Molecular Biology Reagent
Active Motif Preindex Tn5 Transposomes 1x96
Pre-indexed Assembled Tn5 Transposomes are provided in a flexible breakaway 96-well plate format to enable higher throughput ATAC-Seq assays. Each well contains a transposome loaded with a unique i5 and i7 index, enabling direct indexing during tagmentation. By having a unique i7 & i5 Indexed Primer combination in each well, it is easy to perform multiple ATAC-Seq reactions without having to pipette primers separately and keep track of the combinations.
AVIDITY LLC BirA biotin-protein ligase bulk lyophilized reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner. This kit includes 300 ug of BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work.
Gold Biotechnology Inc Pectolyase Y-23 1 g
Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endo-polygalacturonase and endo-pectin lyase in high activity.